SarA influences the sporulation and secondary metabolism in Streptomyces coelicolor M145

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SarA influences the sporulation and secondary metabolism in Streptomyces coelicolor M145.

The filamentous bacteria Streptomyces exhibit a complex life cycle involving morphological differentiation and secondary metabolism. A putative membrane protein gene sarA (sco4069), sporulation and antibiotic production related gene A, was partially characterized in Streptomyces coelicolor M145. The gene product had no characterized functional domains and was highly conserved in Streptomyces. C...

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Role of GntR Family Regulatory Gene SCO1678 in Gluconate Metabolism in Streptomyces coelicolor M145

Here we report functional characterization of the Streptomyces coelicolor M145 gene SCO1678, which encodes a GntR-like regulator of the FadR subfamily. Bioinformatic analysis suggested that SCO1678 is part of putative operon (gnt) involved in gluconate metabolism. Combining the results of SCO1678 knockout, transcriptional analysis of gnt operon, and Sco1678 protein-DNA electromobility shift ass...

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Novel teichulosonic acid from cell wall of Streptomyces coelicolor M145.

The cell wall of Streptomyces coelicolor M145, a prototrophic plasmidless (SCP1(-) SCP2(-)) variant of strain S. coelicolor A3(2) contains the main glycopolymer represented by Kdn-containing teichulosonic acid with unusual structure which has not been described so far: [structure: see text]. The minor polymer was found to be a poly(diglycosyl 1-phosphate) with the following repeating unit: -6)-...

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An unusual response regulator influences sporulation at early and late stages in Streptomyces coelicolor.

WhiI, a regulator required for efficient sporulation septation in the aerial mycelium of Streptomyces coelicolor, resembles response regulators of bacterial two-component systems but lacks some conserved features of typical phosphorylation pockets. Four amino acids of the abnormal "phosphorylation pocket" were changed by site-directed mutagenesis. Unlike whiI null mutations, these point mutatio...

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Time-lapse microscopy of Streptomyces coelicolor growth and sporulation.

Bacteria from the genus Streptomyces are among the most complex of all prokaryotes; not only do they grow as a complex mycelium, they also differentiate to form aerial hyphae before developing further to form spore chains. This developmental heterogeneity of streptomycete microcolonies makes studying the dynamic processes that contribute to growth and development a challenging procedure. As a r...

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ژورنال

عنوان ژورنال: Acta Biochimica et Biophysica Sinica

سال: 2008

ISSN: 1672-9145,1745-7270

DOI: 10.1093/abbs/40.10.877